{"id":155466,"date":"2019-07-12T10:00:25","date_gmt":"2019-07-12T10:00:25","guid":{"rendered":"http:\/\/blog.hannaservice.eu\/mesure-du-ph-de-la-solution-saline-tamponnee-isotonique\/"},"modified":"2022-10-10T12:10:32","modified_gmt":"2022-10-10T12:10:32","slug":"mesure-du-ph-de-la-solution-saline-tamponnee-isotonique","status":"publish","type":"post","link":"https:\/\/blog.hannaservice.eu\/fr\/mesure-du-ph-de-la-solution-saline-tamponnee-isotonique\/","title":{"rendered":"Mesure du pH de la solution saline tamponn\u00e9e isotonique"},"content":{"rendered":"\t\t
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Dans les h\u00f4pitaux, des solutions intraveineuses (IV), telles que du s\u00e9rum physiologique ou des m\u00e9dicaments sur ordonnance, sont couramment administr\u00e9es aux patients pour diverses th\u00e9rapies. Les intraveineuses sont utilis\u00e9es pour administrer des m\u00e9dicaments, remplacer les fluides, \u00e9quilibrer les \u00e9lectrolytes et de nombreux autres traitements.<\/div>\n<\/div>\n
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\u00c9tant donn\u00e9 que les solutions sont introduites directement dans la circulation sanguine, plus la force ionique des solutions est similaire \u00e0 celle du sang, moins il y aura d\u2019impacts n\u00e9gatifs sur la sant\u00e9. La tonicit\u00e9 et le pH sont les deux facteurs les plus importants lors de la fabrication d\u2019une solution qui imite la composition du sang.<\/p>\n

La tonicit\u00e9 et le pH sont les deux facteurs les plus importants lors de la fabrication d\u2019une solution qui imite la composition du sang. La tonicit\u00e9, qui est la pression osmotique d\u2019une solution, fait r\u00e9f\u00e9rence \u00e0 la capacit\u00e9 de l\u2019eau \u00e0 traverser une membrane semi-perm\u00e9able, telle que celle trouv\u00e9e dans une cellule sanguine. L\u2019eau passe de faibles concentrations \u00e0 \u00e9lev\u00e9es. Le mouvement de l\u2019eau de l\u2019int\u00e9rieur d\u2019une cellule sanguine vers le sang est largement bas\u00e9 sur la concentration d\u2019ions dissous dans la solution, appel\u00e9e concentration osmotique.<\/p>\n

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Lorsque des solutions isotoniques (iso = m\u00eame et tonique = force) telles que des solutions salines m\u00e9dicales sont introduites par voie intraveineuse dans la circulation sanguine, il n\u2019y a pas de pression osmotique. Il en r\u00e9sulte une quantit\u00e9 n\u00e9gligeable d\u2019eau entrant et sortant des cellules sanguines.<\/p>\n

Si la solution IV est hypotonique, sa concentration en sel est inf\u00e9rieure \u00e0 celle du sang et des cellules sanguines. La solution saline hypotonique abaissera la concentration d\u2019ions dans le sang et cr\u00e9era un gradient de concentration. Il en r\u00e9sulte que l\u2019eau p\u00e9n\u00e8tre dans les cellules sanguines, o\u00f9 elles gonflent et peuvent se rompre. Les solutions hypertoniques ont une concentration en ions plus \u00e9lev\u00e9e que le sang, et ce gradient de concentration fera sortir l\u2019eau des cellules, les faisant r\u00e9tr\u00e9cir. Lors de la formulation de m\u00e9dicaments IV, des sels tels que le chlorure de sodium et le chlorure de potassium peuvent \u00eatre ajout\u00e9s au m\u00e9dicament actif pour garantir que la solution est isotonique.<\/p>\n

Une autre consid\u00e9ration importante lors de la pr\u00e9paration de m\u00e9dicaments IV est le pH. Le corps humain utilise un syst\u00e8me tampon au bicarbonate qui maintient notre sang \u00e0 un pH presque constant de 7,35. La capacit\u00e9 \u00e0 maintenir l\u2019hom\u00e9ostasie de notre sang est essentielle, car des changements brusques de son pH peuvent entra\u00eener un choc corporel ou un arr\u00eat. Les solutions IV avec des valeurs de pH inf\u00e9rieures \u00e0 5 ou sup\u00e9rieures \u00e0 pH 9 sont connues pour tuer les cellules sanguines au contact, ce qui a un impact n\u00e9gatif important sur la sant\u00e9 des patients. Les meilleurs r\u00e9sultats chez les patients ont \u00e9t\u00e9 observ\u00e9s avec des solutions IV entre un pH de 7,0 \u00e0 7,4. Le pH d\u2019une solution IV affecte \u00e9galement la stabilit\u00e9 du composant m\u00e9dicamenteux actif, ainsi que sa dur\u00e9e de conservation. La plupart des m\u00e9dicaments ont la plus longue stabilit\u00e9 aux valeurs de pH acides. Tenant compte de tout cela, la plupart des solutions m\u00e9dicamenteuses IV sont fabriqu\u00e9es entre pH 5 et pH 6 pour une stabilit\u00e9 maximale tout en garantissant qu\u2019il n\u2019y a pas d\u2019impacts significatifs sur la sant\u00e9. La capacit\u00e9 tampon \u00e9lev\u00e9e de notre pH sanguin garantit qu\u2019il n\u2019y a pas de changements dramatiques lorsque la solution IV s\u2019\u00e9coule dans la circulation sanguine.<\/p>\n

Application<\/h2>\n

Une soci\u00e9t\u00e9 de recherche pharmaceutique a contact\u00e9 Hanna Instruments pour se renseigner sur la mesure du pH de leurs solutions m\u00e9dicamenteuses IV. Ils menaient une \u00e9tude pour d\u00e9terminer la stabilit\u00e9 des solutions m\u00e9dicamenteuses \u00e0 diff\u00e9rentes valeurs de pH, diff\u00e9rentes concentrations de m\u00e9dicament et diff\u00e9rentes tonicit\u00e9s. Comme ils prenaient des mesures fr\u00e9quentes avec un volume d\u2019\u00e9chantillon limit\u00e9, ils effectuaient leurs mesures de pH dans des puits de pipette de 100 L.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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Notre solution<\/h3>\n

Hanna Instruments offert le\u00a0HI5222 pH-m\u00e8tre de paillasse professionnel\/mV\/ISE<\/a>. Ce produit \u00e9tait appropri\u00e9 en raison de la r\u00e9solution du pH de 0,001 et de l\u2019option d\u2019\u00e9talonnage sur des tampons personnalis\u00e9s.<\/p>\n

Pour leurs recherches, la soci\u00e9t\u00e9 calibrait sur deux points, en utilisant les tampons personnalis\u00e9s pour calibrer \u00e0 +\/- 1 unit\u00e9 de pH de leur pH cible. L\u2019entreprise a pu consulter les donn\u00e9es BPL pour s\u2019assurer que la pente et l\u2019\u00e9tat des \u00e9lectrodes \u00e9taient parfaits dans la plage n\u00e9cessaire. Cela leur a assur\u00e9 que leurs mesures \u00e9taient exactes.<\/p>\n

Les donn\u00e9es BPL ont \u00e9galement \u00e9t\u00e9 incluses dans leurs rapports de donn\u00e9es enregistr\u00e9es afin qu\u2019ils puissent facilement se r\u00e9f\u00e9rer et avoir des enregistrements d\u00e9taill\u00e9s de leurs mesures. Le client a appr\u00e9ci\u00e9 le fait que les donn\u00e9es BPL \u00e9taient personnalisables et leur a permis de saisir un \u00ab ID d\u2019op\u00e9rateur \u00bb. Ils ont \u00e9galement utilis\u00e9 l\u2019un des champs \u00ab Informations suppl\u00e9mentaires \u00bb pour inclure un exemple de nom de lot.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Dans les h\u00f4pitaux, des solutions intraveineuses (IV), telles que du s\u00e9rum physiologique ou des m\u00e9dicaments […]<\/p>\n","protected":false},"author":14,"featured_media":4796,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[392],"tags":[],"class_list":["post-155466","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industrie-fr"],"yoast_head":"\nMesure du pH de la solution saline tamponn\u00e9e isotonique - 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