{"id":154398,"date":"2019-07-12T10:00:25","date_gmt":"2019-07-12T10:00:25","guid":{"rendered":"https:\/\/blog.hannaservice.eu\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\/"},"modified":"2022-10-10T09:01:29","modified_gmt":"2022-10-10T09:01:29","slug":"odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook","status":"publish","type":"post","link":"https:\/\/blog.hannaservice.eu\/sr\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\/","title":{"rendered":"Odre\u0111ivanje pH izotoni\u010dnog puferskog rastvora Share on linkedin Share on facebook"},"content":{"rendered":"\t\t
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U bolnicama se pacijentima \u010desto daju intravenski (IV) rastvori za razli\u010dite terapije, poput fiziolo\u0161kih rastvora i raznih terapija koje se prepisuju na recept. Intravenski se daju razni lekovi, terapije, vr\u0161i zamena te\u010dnosti, uravnote\u017eenje elektrolita i mnogi drugi tretmani.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Po\u0161to se rastvori daju direktno u krvotok, \u0161to je njihova jonska ja\u010dina sli\u010dnija krvi, manja je verovatnoc\u0301a da c\u0301e doc\u0301i do negativnih uticaja na zdravlje. Osmotski pritisak i pH su dva najva\u017enija faktora prilikom pravljenja rastvora koji opona\u0161a sastav krvi.<\/p>\n

Osmotski pritisak i pH su dva najva\u017enija faktora prilikom pravljenja rastvora koji opona\u0161a sastav krvi. Osmotski pritisak rastvora, odnosi se na sposobnost vode da pro\u0111e kroz polupropusnu membranu, kao \u0161to je ona u krvnoj c\u0301eliji. Voda se kre\u0107e od manjih ka vi\u0161im koncentracijama. Kretanje vode iz krvne c\u0301elije u krv u velikoj se meri zasniva na koncentraciji rastvorenih jona u rastvoru, koja se naziva osmotskom koncentracijom<\/p>\n

Kada se izotoni\u010dni (iso = isti i tonic = snaga) rastvori, poput medicinskih fiziolo\u0161kih rastvora, intravenski uvode u krvotok, ne postoji osmotski pritisak. To rezultira neznatnom koli\u010dinom vode koja se krec\u0301e u krvne c\u0301elije i iz njih.<\/p>\n

Ako je rastvor IV hipotoni\u010dan, koncentracija soli je ni\u017ea nego u krvi i krvnim c\u0301elijama. Hipotonski fiziolo\u0161ki rastvor c\u0301e sniziti koncentraciju jona u krvi. To dovodi do toga da se voda krec\u0301e u krvne c\u0301elije, gde dolazi do oticanja i mogu\u0107eg pucanja. Hipertonski rastvori imaju vec\u0301u koncentraciju jona od krvi, \u0161to dovodi do odliva vode iz \u0107elija i time ih smanjuju. Kada se proizvode izotonskii lekovi, soli kao \u0161to su natrijum hlorid i kalijum hlorid mogu se dodati aktivnom leku kako bi se obezbedilo da rastvor bude izotoni\u010dan.<\/p>\n

Druga va\u017ena stavka za razmatranje prilikom pripreme izotonskih lekova je pH. Ljudsko telo koristi sistem bikarbonata koji odr\u017eava na\u0161u krv na gotovo konstantnom pH od 7,35. Sposobnost odr\u017eavanja homoeostaze na\u0161e krvi je kriti\u010dna, jer nagle promene njegovog pH mogu dovesti do telesnog \u0161oka ili \u010dak prekida rada. Poznato je da izotonski rastvori sa vrednostima ni\u017eim od pH 5 ili vec\u0301im od pH 9 ubijaju krvne c\u0301elije pri kontaktu, \u0161to ima zna\u010dajno negativni uticaj na zdravlje pacijenata. Najbolji rezultati za pacijente se posti\u017eu kada rastvor ima pH 7.0 \u2013 7.4. pH vrednosti izotonskih rastvora tako\u0111e uti\u010de na stabilnost aktivne komponente leka, kao i na njegov rok trajanja. Ve\u0107ina lekova su du\u017ee stabilna pri kiselijim vrednostima pH. Uzimajuc\u0301i sve to u obzir, vec\u0301ina IV lekova se proizvodi izme\u0111u pH 5 i pH 6 za maksimalnu stabilnost, spre\u010davaju\u0107i tako zna\u010dajne uticaje na zdravlje. Puferski kapacitet na\u0161e krvi obezbe\u0111uje protok izotopskih rastvora kroz krv bez dramati\u010dnih promena.<\/p>\n

Primena<\/h2>\n

Farmaceutska istra\u017eiva\u010dka kompanija kontaktirala je kompaniju Hanna Instruments kako bi se raspitali o nabavci pH mera\u010da za testiranje izotopskih rastvora. Obavljali su studiju za utvr\u0111ivanje stabilnosti rastvora pri razli\u010ditim pH vrednostima, razli\u010ditim koncentracijama lekova i razli\u010ditim osmotskim pritiscima. Po\u0161to su vr\u0161ili \u010desta merenja sa ograni\u010denom zapreminom uzorka, izvodili su pH merenja u 100 mL pipetama.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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Na\u0161e re\u0161enje<\/h3>\n

Hanna je ponudila\u00a0HI5222 profesionalni, laboratorijski pH\/mV\/ISE mera\u010d<\/a>. Ovaj proizvod je bio pogodan zbog pH rezolucije 0,001 i moguc\u0301nosti kalibracije sa prilago\u0111enim puferima.<\/p>\n

Za svoje istra\u017eivanje, kompanija je kalibrisala u dve ta\u010dke, koristec\u0301i prilago\u0111ene pufere za kalibraciju do +\/- 1 pH jedinice svog ciljanog pH. Kompanija je bila u moguc\u0301nosti da pogleda GLP podatke kako bi se uverila da su nagib i stanje elektrode idealni unutar njihovog potrebnog raspona. To ih je uveravalo da su njihova merenja ta\u010dna.<\/p>\n

Podaci o GLP-u tako\u0111e su uklju\u010deni u izve\u0161taje o evidentiranim podacima kako bi mogli da se lako upute nazad i imaju detaljne zapise o merenjima. Kupac se posebno dopalo \u0161to su GLP podaci prilagodljivi i to \u0161to je mogu\u0107e uno\u0161enje \u201eID-a operatera\u201c. Tako\u0111e su koristili \u201cAdditional Info\u201d kako bi ozna\u010davali serije uzoraka.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

U bolnicama se pacijentima \u010desto daju intravenski (IV) rastvori za razli\u010dite terapije, poput fiziolo\u0161kih rastvora […]<\/p>\n","protected":false},"author":17,"featured_media":4798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[367],"tags":[],"class_list":["post-154398","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industrija"],"yoast_head":"\nOdre\u0111ivanje pH izotoni\u010dnog puferskog rastvora Share on linkedin Share on facebook - Hanna Instruments Srbja<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/blog.hannaservice.eu\/sr\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\/\" \/>\n<meta property=\"og:locale\" content=\"sr_RS\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Odre\u0111ivanje pH izotoni\u010dnog puferskog rastvora Share on linkedin Share on facebook - Hanna Instruments Srbja\" \/>\n<meta property=\"og:description\" content=\"U bolnicama se pacijentima \u010desto daju intravenski (IV) rastvori za razli\u010dite terapije, poput fiziolo\u0161kih rastvora […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/blog.hannaservice.eu\/sr\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\/\" \/>\n<meta property=\"og:site_name\" content=\"Hanna Instruments Srbja\" \/>\n<meta property=\"article:published_time\" content=\"2019-07-12T10:00:25+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2022-10-10T09:01:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/blog.hannaservice.eu\/wp-content\/uploads\/2021\/03\/hs-buffer-saline.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"800\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Alan Curtis\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Napisano od\" \/>\n\t<meta name=\"twitter:data1\" content=\"Alan Curtis\" \/>\n\t<meta name=\"twitter:label2\" content=\"Procenjeno vreme \u010ditanja\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minuta\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\\\/\"},\"author\":{\"name\":\"Alan Curtis\",\"@id\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/#\\\/schema\\\/person\\\/675987b8b5fd7dfbc3ccca6b0bd2d486\"},\"headline\":\"Odre\u0111ivanje pH izotoni\u010dnog puferskog rastvora Share on linkedin Share on facebook\",\"datePublished\":\"2019-07-12T10:00:25+00:00\",\"dateModified\":\"2022-10-10T09:01:29+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\\\/\"},\"wordCount\":698,\"publisher\":{\"@id\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/blog.hannaservice.eu\\\/wp-content\\\/uploads\\\/2021\\\/03\\\/hs-buffer-saline.jpg\",\"articleSection\":[\"Industrija\"],\"inLanguage\":\"sr-RS\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\\\/\",\"url\":\"https:\\\/\\\/blog.hannaservice.eu\\\/sr\\\/odredjivanje-ph-izotonicnog-puferskog-rastvora-share-on-linkedin-share-on-facebook\\\/\",\"name\":\"Odre\u0111ivanje pH izotoni\u010dnog puferskog rastvora Share on linkedin Share on facebook - 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