{"id":154549,"date":"2020-07-08T10:10:06","date_gmt":"2020-07-08T10:10:06","guid":{"rendered":"https:\/\/blog.hannaservice.eu\/testiranje-vina-8-saveta-kako-da-pripremite-laboratoriju\/"},"modified":"2022-10-10T08:56:39","modified_gmt":"2022-10-10T08:56:39","slug":"testiranje-vina-8-saveta-kako-da-pripremite-laboratoriju","status":"publish","type":"post","link":"https:\/\/blog.hannaservice.eu\/sr\/testiranje-vina-8-saveta-kako-da-pripremite-laboratoriju\/","title":{"rendered":"Testiranje vina \u2013 8 saveta kako da pripremite laboratoriju"},"content":{"rendered":"\t\t
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Poznato je da je dobijanje ta\u010dnih i pouzdanih merenja od presudnog zna\u010daja u proizvodnji vina. Poslednje \u0161to je potrebno da se desi tokom rada u va\u0161oj vinskoj laboratoriji je da shvatite da na pola puta ne\u0161to nedostaje ili da neki deo opreme ne radi pravilno.<\/p>\n

Iz toga razloga sastavili smo ovaj kratki vodi\u010d kroz pripremu vinske laboratorije za testiranje vina. Pratec\u0301i ovih osam jednostavnih koraka, mo\u017eete biti sigurni da \u0107e va\u0161a vinska laboratorija biti uvek spremna da podnese sve Va\u0161e potrebe za testiranjem, uklju\u010dujuc\u0301i pH, SO2<\/sub>, ORP, ukupnu kiselost i refraktometrijska testiranja.<\/p>\n

    \n
  1. Ne zaboravite na rastvore \u2013 proverite zalihe!<\/li>\n
  2. Upotreba odgovaraju\u0107ih rastvora za \u010di\u0161\u0107enje elektrode<\/li>\n
  3. Upotreba rastvora za skladi\u0161tenje elektrode<\/li>\n
  4. Proverite nivo elektrolita<\/li>\n
  5. Uverite se da elektrode funkcioni\u0161tu ispravno<\/li>\n
  6. Nabavite rezervnu elektrodu<\/li>\n
  7. Razmislite o mini titratoru<\/li>\n
  8. Nadogradite refraktometrsijska merenja digitalnim refraktometrom<\/li>\n<\/ol>\n

    1. Ne zaboravite na rastvore \u2013 proverite zalihe!<\/h2>\n

    pH elektrode treba kalibrisati pre upotrebe. U idealnom slu\u010daju, treba vr\u0161iti svakodnevne kalibracije kako bi merenja bila ta\u010dna. Vremenom c\u0301e se otvorena boca pufera razgraditi, a pH vrednost nazna\u010dena na ambala\u017ei nec\u0301e biti stvarna pH vrednost rastvora. Iz ovog razloga trebalo bi da zamenite bilo koji pufer koji je otvoren pre vi\u0161e od \u0161est meseci, bez obzira na datum isteka paketa.<\/p>\n

    Od\u0161tampajte na\u0161 vodi\u010d kroz najbolje prakse pH merenja vina, kako biste odgovaraju\u0107i podsetnik. Popunite kontakt formu na sajtu i mi \u0107emo Vam poslati vodi\u010d besplatno.<\/p>\n

    Naj\u010de\u0161\u0107e kori\u0161\u0107eni kalibracioni puferi koji se koriste za testiranje vina su pH 7.01 pufer (500 ml) \u2013 HI7007L, pH 4.01 pufer (500 ml) \u2013 HI7004L, i pH 3.00 pufer (500 ml) \u2013 HI5003.<\/p>\n

    <\/p>\n

    2. Upotreba odgovaraju\u0107ih rastvora za \u010di\u0161\u0107enje elektrode<\/h2>\n

    Kako bi funkcionisale nesmetano elektrode je potrebno valjano o\u010distiti. Mrlje od vina na elektrodi, vinski ostaci i naslage na senzornom delu elektrode prouzrokova\u0107e promenu generisane volta\u017ee izme\u0111u pH elektrode i rastvora. Tako\u0111e, potrebno je obratiti pa\u017enju na za\u010depljivanje spoja elektrode. Naslage na staklu elektrode ili za\u010depljenja spoja izazivaju usporavanje o\u010ditanja i ugro\u017eenu preciznost. Preporu\u010dujemo upotrebu HI1048 elektrode koja je specijalno dizajnirana da omogu\u0107i najbolje performanse prilikom testiranja vina.<\/p>\n

    Hanna savet:<\/strong>\u00a0Koristite specijalno formulisane rastvore za otklanjanje vinskih mrlja i naslaga. Za vinske namene preporu\u010dujemo upotrebu rastvora za \u010di\u0161\u0107enje od vinskih ostataka (500mL) \u2013 HI70635L ili rastvor za \u010di\u0161\u0107enje elektrode od vinskih mrlja (500 mL) \u2013 HI70636L.<\/p><\/blockquote>\n

    3. Upotreba rastvora za skladi\u0161tenje elektrode<\/h2>\n

    Rastvori za skladi\u0161tenje\/\u010duvanje pH elektrode kao \u0161to je na\u0161 HI 70300L od 500 mL su dizajnirani tako da obezbe\u0111uju da pH elektroda ostane hidrirana i odr\u017ei nivo protoka elektrolita. Nakon \u0161to o\u010distite elektrodu, potrebno je da je hidrirate u rastvoru za skladi\u0161tenje na bar 3-4 sata i elektrodu bi nakon toga trebalo \u010duvati u istom rastvoru kada je ne koristite.<\/p>\n

    Osu\u0161eni spoj usporava protok elektrolita unutar elektrode. To uti\u010de na potencijal spoja, kao i na stabilnost i odziv elektrode. Pravilno uskladi\u0161tene elektrode pokazuju vec\u0301u ta\u010dnost i du\u017ei vek trajanja od onih koje se ne skladi\u0161te pravilno.<\/p>\n

    Hanna savet:<\/strong>\u00a0Da li imate elektrodu koja je pravilno skladi\u0161tena, a ne pokazuje rezultate kako bi trebalo? Preuzmite na\u0161 vodi\u010d za proveru perfomasni pH elektrode.<\/p><\/blockquote>\n

    4. Proveritte nivo elektrolita<\/h2>\n

    PH ili ORP elektrode koje su punjive potrebno je dopuniti elektrolitom u odre\u0111enom trenutku. Elektroda mora stalno imati odgovaraju\u0107i nivo elektrolita kako bi se obezbedila odgovaraju\u0107i pritisak. Ovaj pritisak omoguc\u0301ava protok elektrolita kroz spoj i uzorak. Joni u elektrolitu ispunjavaju elektri\u010dno merno kola izme\u0111u mera\u010da, elektrode i uzorka vina koji se ispituje.<\/p>\n

    <\/p>\n

    Hanna savet<\/strong>: Uklonite poklopac prilikom testiranja kako biste obezbedili protok. Zamenite elektrolit i zategnite poklopac kako biste obezbedili elektrodu tokom skladi\u0161tenja.<\/p><\/blockquote>\n

    Elektrode sa jednim i dva spajanja imaju razli\u010dite rastvore za punjenje<\/strong><\/h3>\n

    Ne znate kakav spoj ima va\u0161a elektroda? Dijagram ispod Vam mo\u017ee pomo\u0107i da prepoznate vrstu spoja. Koristite na\u0161\u00a0<\/strong>HI7082 rastvor ako imate elektrodu sa dvostrukim spajanjem ili HI7071 rastvor ukoliko je jednostruki spoj.<\/p>\n

    <\/p>\n

     <\/p>\n

    5. Uverite se da elektrode funkcioni\u0161tu ispravno<\/h2>\n

    pH Elektrode<\/h3>\n

    Elektrode imaju ograni\u010den \u017eivotni vek. O\u010ditavanje koje traje predugo da bi se stabilizovalo (du\u017ee od 30 sekundi) mo\u017ee biti znak da vam elektroda propada.<\/p>\n

    Za proveru ukupnog stanja pH elektrode potrebno je proveriti dve vrednosti: pomak (mV vrijednost u pH 7,01) i nagib (mV razlika izme\u0111u pH 7,01 i pH 4,01). Pomak (offset) i nagib pH elektrode uvek treba kontrolisati sve\u017eim puferima. Ako va\u0161 mera\u010d ima opciju Dobra laboratorijska praksa (GLP), on c\u0301e Vam dostaviti podatke nakon kalibracije.<\/p>\n

    Ukoliko Va\u0161 mera\u010d nema GLP opciju, bilo koji mera\u010d sa mV re\u017eimom mo\u017ee se koristiti za proveru pH elektrode.<\/p>\n

    Evo uputstava za proveru nagiba i pomaka za mera\u010de bez GLP re\u017eima:<\/p>\n

    Pomak<\/h4>\n

    Teoretski kada posatavite pH elektrodu u rastvor pH 7.01, mV vrednost bi trebalo da bude 0. Me\u0111utim, zbog varijacija u staklu, starenju i kondicioniranju, offset napon c\u0301e obi\u010dno nestati, tako da je prihvatljivi offset napon +\/- 30 mV. Vec\u0301ina mera\u010da omogu\u0107ava kalibraciju na vrednostima od +\/- 60 mV za pufer pH 7.01, ali u ovom trenutku ta\u010dnost merenja c\u0301e se smanjiti.<\/p>\n

    Nagib<\/h4>\n

    Idealan nagib pH elektrode je 100% odnosno 59.16 mV promene na 1 pH jedinicu @ 25 \u00baC. Za pH 4, to je 177.48 mV razlika, jer je pH 4 pufer 3 jedinice pH udaljen od ta\u010dke pH 7 (59.16 mV x 3). Apsolutni minimalni nagib operativne elektrode je 85% ili 150 mV (177,48 k 0,85).<\/p>\n

    Ako su vrednosti pomaka i nagiba izvan prihvatljivih raspona, poku\u0161ajte da o\u010distite i hidrirate elektrodu, a zatim ponovo testirajte. Za punjive elektrode mo\u017ee se preduzeti dodatni korak u vidu zamene elektrolita. Ukoliko su vrednosti i dalje van opsegu, Va\u0161oj elektrodi je potrebna zamena.<\/p>\n

    ORP Elektrode<\/h3>\n

    U potenciometrijskim titracijama za odre\u0111ivanje SO2<\/sub>\u00a0koriste se ORP elektrode za odre\u0111ivanje zavr\u0161ne ta\u010dke titracije. Va\u017eno je povremeno proveriti ORP elektrodu pomoc\u0301u ORP test rastvora.<\/p>\n

    Da bi se proverila ORP elektroda, sonda se sme\u0161ta u ORP test rastvor poznate vrednosti. Uzmimo HI7021L 240 mV @ 25\u00b0C ORP Test rastvor (500 mL) kao primer. Ispravno funkcioni\u0161u\u0107a elektroda \u010dita\u0107e 240 mV +\/- 50 mV. O\u010ditavanja izvan ovog raspona pokazuju da vrh platine i \/ ili traku treba polirati, ili da se mora promeniti rastvor za punjenje elektrolita. Ako se ORP elektroda ne mo\u017ee dovesti u domet, onda je treba zameniti.<\/p>\n

    Hanna savet<\/strong>: Vrlo fini glas-papir, (kao 2000), mo\u017ee se koristiti za poliranje zamrljanog vrha ili ORP-a. ORP senzorski deo elektrode treba da bude sjajan.<\/p><\/blockquote>\n

    6. Nabavite rezervnu elektrodu<\/h2>\n

    Zbog ograni\u010denog \u017eivotnog veka elektroda, preporu\u010dujemo vam da imate zamensku elektrodu.<\/p>\n

    Toplo preporu\u010dujemo upotrebu namenskih elektroda za vino. Faktori kao \u0161to su vrsta tela, materijal za spajanje, vrsta spoja, rastvor za punjenje i vrsta stakla, svi su deo procesa dizajniranja. Prava kombinacija materijala i tehnologija zna\u010dajno pobolj\u0161ava ta\u010dnost, jednostavnost upotrebe i radni vek elektrode.<\/p>\n

    Vrste i prednosti vinskih specifi\u010dnih elektroda<\/h3>\n

    Serija elektroda HI1048 dizajnirana je sa posebnim karakteristikama koje specijalno koriste vinari.<\/p>\n

    CPS spoj sa za\u0161titnim rukavom<\/h4>\n

    HANNIN CPS sistem za prevenciju za\u010depljenja je inovacija u svetu elektroda. Konvencionalne pH elektrode koriste se kerami\u010dke spojeve koje se brzo za\u010depljuju kada se koriste sa uzorcima vina. Kada je za\u010depljen spoj, elektroda ne funkcioni\u0161e. CPS tehnologija koristi porozno staklo u kombinaciji sa PTFE (materijalom sli\u010dnim Teflonu \u2122) i za\u0161titni rukav koji treba da spre\u010di za\u010depljenje; porozno staklo omoguc\u0301ava pravilan protok elektrolita, dok PTFE odbija prljav\u0161tinu. Kao rezultat toga, pH elektrodama sa CPS c\u0301e trebati manje odr\u017eavanja i funkcioni\u0161u du\u017ee nego \u0161to to uobi\u010dajena elektroda koristi u proizvodnji vina.<\/p>\n

    <\/p>\n

     <\/p>\n

    Dupli referentni spoj<\/h4>\n

    Elektroda sa dvostrukim spajanjem, kao \u0161to je elektroda HI1048 iz vinske serije, ima unutra\u0161nji odeljak koji okru\u017euje referentnu \u017eicu. Joni srebra prisutni su u elektrolitu unutra\u0161njeg odeljka, u kome se nalazi referentna \u017eica; elektrolit izvan ovog odeljka ne sadr\u017ei srebro. Dizajn dvostrukog spoja zna\u010di da se uzorak ne kontaminira srebrom. Ovaj dizajn omoguc\u0301ava merenje u primenama gde su joni srebra u uzorku nepo\u017eeljni.<\/p>\n

    Priklju\u010dci elektroda<\/h4>\n

    Serija elektroda HI1048 dolazi na raspolaganju sa raznim priklju\u010dcima, tako da se mogu koristiti sa \u0161irokim rasponom instrumenata. Dva najpopularnija priklju\u010dka su BNC i 3.5mm priklju\u010dak.<\/p>\n

    <\/p>\n

     <\/p>\n

    BNC priklju\u010dci \u2013 kao kod pH elektrode sa CPS i BNC konektorom \u2013 HI1048B \u2013 su univerzalni i mogu se koristiti sa bilo kojim mera\u010dem koji ima BNC konektor, kao \u0161to je Mini-titrator za merenje titrabilne kiseline u vinu \u2013 HI84502.<\/p>\n

    <\/p>\n

    3,5 mm pin priklju\u010dak, poput onog na digitalnoj staklenoj pH elektrodi sa CPS \u2013 HI10480 za edge mera\u010d, je digitalna pH elektroda koja sadr\u017ei ugra\u0111eni mikro\u010dip koji \u010duva informacije kao \u0161to su tip senzora, serijski broj i informacije o kalibraciji, uklju\u010dujuc\u0301i datum, vreme, pomak, nagib, stanje sonde i kori\u0161c\u0301ene pufere. HI10480 ima ugra\u0111eni senzor za merenja kompenzovana temperaturom.<\/p>\n

    Ova vrsta priklju\u010dka mo\u017ee se koristiti samo s linijom mera\u010da edge, koja uklju\u010duje edge za odre\u0111ivanje pH vina \u2013 2020W,edge namenski pH \/ ORP \u2013 HI2002, edge Multiparametar pH \u2013 HI2020, edge Multiparametar EC \/ TDS \/ Salinitet \u2013 HI2030, i edge vi\u0161eparametarski mera\u010d rastvorenog kiseonika \u2013 HI2040.<\/p>\n

    Namenska pH elektroda za vino sa CPS \u2013 HI10480 standardno se isporu\u010duje sa HI2020w kompletom za merenje pH vrednosti vina.<\/p>\n

    HALO be\u017ei\u010dna pH elektroda<\/h4>\n

    Bluetooth HALO pH elektroda za vino \u2013 HI10482 nudi sve karakteristike elektrode HI1048, istovremeno prave\u0107i u\u0161tede za nabavku pH mera\u010da. Nakon \u0161to se besplatna aplikacija Hanna Lab preuzme na Va\u0161 pametni telefon ili tablet, Va\u0161 pametni ure\u0111aj postaje profesionalni pH mera\u010d!<\/p>\n

    <\/p>\n

     <\/p>\n

    Karakteristike aplikacije Hanna Lab uklju\u010duju laku kalibraciju, merenje pH, evidentiranje podataka, grafikovanje i deljenje podataka. HALO nudi odli\u010dan dodatak va\u0161em kompletu alata za analizu pH vrednosti i mo\u017ee poslu\u017eiti kao rezervni ili kao dodatni mera\u010d za periodi\u010dne analize velike zapremine.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

    Poznato je da je dobijanje ta\u010dnih i pouzdanih merenja od presudnog zna\u010daja u proizvodnji vina. 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